Basic hands-on introduction to holography for ophthalmology and optometry undergraduate students

The three-dimensional visualization of objects and structures is very important in a number of situations also because that is how we normally see. Holography is a well-established way of creating 3D images of real objects. The technical and scientific developments over last decades in this field turn holography, either analogue and digital, a very powerful tool in many different applications including in medicine. Important advances were made also in domains involving eyesight and the eye like optometry, ophthalmology and ophthalmic optics

Breast cancer detection using microwave holography

Breast cancer is one of the most common forms of cancer in women. X-ray mammography is the most widely used technique for early detection but has limitations. In this paper, an alternative approach for breast cancer detection using microwave imaging is presented. This is based upon microwave indirect holographic approach, central to which is the use of a synthetic reference beam. This approach has benefits in terms of simplicity and expense. Experimental results using a simple breast phantom are included to demonstrate the potential of this approach

Field-portable pixel super-resolution colour microscope.

Based on partially-coherent digital in-line holography, we report a field-portable microscope that can render lensfree colour images over a wide field-of-view of e.g., >20 mm(2). This computational holographic microscope weighs less than 145 grams with dimensions smaller than 17×6×5 cm, making it especially suitable for field settings and point-of-care use. In this lensfree imaging design, we merged a colorization algorithm with a source shifting based multi-height pixel super-resolution technique to mitigate 'rainbow' like colour artefacts that are typical in holographic imaging. This image processing scheme is based on transforming the colour components of an RGB image into YUV colour space, which separates colour information from brightness component of an image. The resolution of our super-resolution colour microscope was characterized using a USAF test chart to confirm sub-micron spatial resolution, even for reconstructions that employ multi-height phase recovery to handle dense and connected objects. To further demonstrate the performance of this colour microscope Papanicolaou (Pap) smears were also successfully imaged. This field-portable and wide-field computational colour microscope could be useful for tele-medicine applications in resource poor settings